白菜型油菜DFR基因的克隆与序列分析

By using RACE (rapid amplification ofcDNA ends) based homologous cloning strategy, we have successfully isolated the genomic and full-length cDNA sequences of a gene encoding typical DFR (dihydroflavonol-4-reductase) from black-seeded Brassica campestris L. var. oleifera DC.. The gene, designated BcDFR here, is 1 722bp in length and harbors 5 introns with typical splice sites of plant DFR genes. BcDFR cDNA is 1311bp in length with a 1 158bp ORF as well as a 25bp 5‘ UTR and a 128bp 3‘ UTR. The encoded BcDFR protein is 385 aa with a calculated Mw of 42.85kD and a pI value of 5.55. The nucleotide and amino acid sequences of this gene share extensive homologies to plant DFR genes of wide origins especially high similarities to Cruciferous DFR genes. Sequence analyses such as phylogenetic analysis, conserved domain search and substrate specificity region detection all indicated that BcDFR gene is a quite potentially biofunctional gene. Its cloning enables us to further dissect the possible relatedness between DFR gene and Brassica seed coat color traits and to create transgenic novel yellow-seeded rapeseed germplasm through antisense- or RNAi-suppression of DFR gene expression in black-seeded elite cultivars.     

RECREATING SMALL SEAMLESS TUBE MILLS BY PIERCING INSFEAD OF ROLLING

This paper discuss the effects of automatic plug mills in small seamless tube mills. Far from traditional ideas,the auther comes rp with mew ideas, which adot the mew process of replacing rolling by piercing,a huge sum of economical interests and great social benefits will be gained.     

An Expert Knowledge-acquisition Method on Line of Automatic Excluding Errors

Aimed at soving the problem existing in the knowledge-acquisition directing-operation expert systems, a new method is proposed to acquire knowledge of expert-operation, excluding errors existed in examples and reinforce the accuracy of examples,the goal of knowledge acquisition can be achieved, the method presented in this paper solves the problem of self-enhancement of expert knowledge on line efficiently.     

A Kind of Directing Operation Expert Systems Based on Self-adaptive Predictor

In the general directing-operation expert systems.the information of directingoperation is often shown after the subprocess has finished,The aim of the thesis is to enhance thefunction of directing-operation,and suggest that we add the self-tuning predictor in the expertsystems and use it to direct the present operation.We have shown the case oftemperature-predicting experiment made in the small wine distillatorv so that we can illustrate thatit is feasible to use the predictor to direct the operation.     

地源热泵技术在中国温室设施中的应用探讨

当前在中国温室设施中应用和推广地源热泵技术尚存在诸多不利因素,大大限制了该项技术在设施农业领域中的发展。为探寻合理的解决途径,对地源热泵空调系统的关键技术和其在中国的发展概况进行了相关的阐述和分析,并以中国农业大学上庄实验站已经建成使用的地下水式温室地源热泵空调系统为实例,介绍该系统的组成、工作流程、性能参数和使用情况,探讨温室地源热泵空调系统在推广和使用中可能出现的投资较高,长时间运行性能不稳定等问题,分析其原因并提出相应的对策建议。     

农产品加工中的营养学思考与营养食品生产

中国农产品生产及其加工曾经经历过注重产品数量扩增、技术设备改进和卫生安全的发展阶段,目前正处在挑战和发展机遇并存的转折时期.中国农产品生产及其加工业应把握时机,应用营养科学为指导,科学地制定"十一五"期间的发展战略,丰富和发展营养食品产业,促进农产品生产和加工全面满足人民营养健康需要和市场需求,这将是开拓发展领域,实现产品升级换代,提升竞争能力和增值潜力,主动迎接国际挑战的战略举措,也是实践"以人为本"的科学发展观的具体体现.     

用Delphi实现温度传感器的数据采集和误差分析

利用Delphi嵌入式汇编的扩展功能实现基于I／O的热敏电阻温度传感器数据采集，并用二次样条插值法研究电压和温度特性非线性化的S曲线，减少非线性误差。     

不同产地瓦松活性成分初步研究

目的:讨论不同产地瓦松提取物活性成分差异,以期为瓦松应用开发提供理论基础。方法:采用系统化学检测法研究瓦松水提物及醇提取物成分差异;采用HPLC法,对水提物正丁醇萃取相进行了讨论,研究其差异。结果:不同产地瓦松水提物及醇提物存在一定差异。结论:相对来说辽宁、黑龙江所产瓦松活性成分较为丰富,相对含量较高。     

Multi‐channel visual evoked potential as an ancillary tool to diagnose intraorbital optic nerve lesions

Multi‐channel visual evoked potential (MVEP) recording method was used to assist in diagnosing a 4‐month‐old Chinese Albino rabbit with an intraorbital mass. Subcutaneous MVEP of its both eyes were recorded simultaneously using 16 electrodes (4 × 4) multi‐channel array. Analysis of the cortical potential landscapes (CPL) showed that the conduction function of right eye was remarkably impaired in terms of decreased amplitudes and prolonged latencies. Specific side‐dominant distribution asymmetry of the decreased MVEP amplitudes indicated that the temporal side of the optic nerve (ON) was severely involved. Overall prolonged latencies of the CPL without side differences suggested that the functional impairment could have been caused by the mechanical compression exerted by an intraorbital mass. Surgical removal procedures confirmed that the mass was located temporally to the ON. Pathological examination provided a final diagnosis of a giant polycystic mucocele. Beyond its significance as a standard tool to assess functional changes of the visual pathway, MVEP recordings might assist locating intraorbital lesions that involve the ON by careful analysis of abnormal CPLs.